dihydroethidium dhe superoxide anion fluorescent probe kit Search Results


superoxide red fluorescent probe  (Yeasen Biotechnology)
86
Yeasen Biotechnology superoxide red fluorescent probe
Superoxide Red Fluorescent Probe, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellular ros superoxide detection assay kit  (Abcam)
99
Abcam cellular ros superoxide detection assay kit
Cellular Ros Superoxide Detection Assay Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellular ros superoxide detection assay kit - by Bioz Stars, 2026-11
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superoxide anion fluorescent probe dihydroethidium  (MedChemExpress)
98
MedChemExpress superoxide anion fluorescent probe dihydroethidium
Superoxide Anion Fluorescent Probe Dihydroethidium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superoxide anion fluorescent probe dihydroethidium - by Bioz Stars, 2026-11
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ros  (Dojindo Labs)
95
Dojindo Labs ros
Impact of various antioxidants on fresh bull sperm motility and reactive oxygen species <t>(ROS)</t> levels when exposed to different oxidative stress triggers. ( A ) Experimental design to examine the impact of various antioxidants on sperm motility and levels of reactive oxygen species (ROS) under the two oxidative stress inducers. ( B and C ) Changes in sperm viability and motility under H 2 O 2 ( B ) or antimycin (C). ( D - G ) Effect of antioxidants on the percentages <t>of</t> <t>DCFH-DA</t> and mtSOX deep Red positive sperm under H 2 O 2 or antimycin. ( H - J ) Measurement of mitochondrial activity using the JC-1 kit. ( H ) Representative flow cytometric pattern of sperm that was not treated with any drug (control) stained with JC-1. Effect of antioxidants on the percentages of high mitochondrial membrane potential (hMMP) under the treatment of H 2 O 2 ( I ) and antimycin ( J ). H 2 O 2 or antimycin groups were used as controls for statistical analysis ( p < 0.05).
Ros, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dihydroethidium+dhe+superoxide+anion+fluorescent+probe+kit/ROS+Assay+Kit+-Photo-oxidation+Resistant+DCFH-DA/pmc11534973-51-7-20
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ros - by Bioz Stars, 2026-11
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dihydroethidium dhe superoxide anion fluorescent probe kit  (Servicebio Inc)
86
Servicebio Inc dihydroethidium dhe superoxide anion fluorescent probe kit
BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) <t>fluorescent</t> probe, accompanied by the relevant quantitative determination. ( b , d ) <t>Superoxide</t> anion (O 2 − ) production in irradiated BMSCs was monitored with the <t>DHE</t> fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.
Dihydroethidium Dhe Superoxide Anion Fluorescent Probe Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dihydroethidium+dhe+superoxide+anion+fluorescent+probe+kit/fast+green+plant+solution+staining/pmc13113114-166-9-17
Average 86 stars, based on 1 article reviews
dihydroethidium dhe superoxide anion fluorescent probe kit - by Bioz Stars, 2026-11
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mitoros 580  (AAT Bioquest)
90
AAT Bioquest mitoros 580
BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) <t>fluorescent</t> probe, accompanied by the relevant quantitative determination. ( b , d ) <t>Superoxide</t> anion (O 2 − ) production in irradiated BMSCs was monitored with the <t>DHE</t> fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.
Mitoros 580, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dihydroethidium+dhe+superoxide+anion+fluorescent+probe+kit/mitoros+580/pmc10074111-283-6-12
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dihydroethidium  (Yeasen Biotechnology)
86
Yeasen Biotechnology dihydroethidium
BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) <t>fluorescent</t> probe, accompanied by the relevant quantitative determination. ( b , d ) <t>Superoxide</t> anion (O 2 − ) production in irradiated BMSCs was monitored with the <t>DHE</t> fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.
Dihydroethidium, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dihydroethidium+dhe+superoxide+anion+fluorescent+probe+kit/dihydroethidium/pm38679470-85-7-13
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dihydroethidium - by Bioz Stars, 2026-11
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total ros/superoxide detection kit  (Enzo Biochem)
90
Enzo Biochem total ros/superoxide detection kit
BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) <t>fluorescent</t> probe, accompanied by the relevant quantitative determination. ( b , d ) <t>Superoxide</t> anion (O 2 − ) production in irradiated BMSCs was monitored with the <t>DHE</t> fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.
Total Ros/Superoxide Detection Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dihydroethidium+dhe+superoxide+anion+fluorescent+probe+kit/total+ros+superoxide+detection+kit/pm35486520-67-14-18
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total ros/superoxide detection kit - by Bioz Stars, 2026-11
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dihydroethidium  (Macklin Inc)
86
Macklin Inc dihydroethidium
BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) <t>fluorescent</t> probe, accompanied by the relevant quantitative determination. ( b , d ) <t>Superoxide</t> anion (O 2 − ) production in irradiated BMSCs was monitored with the <t>DHE</t> fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.
Dihydroethidium, supplied by Macklin Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dihydroethidium+dhe+superoxide+anion+fluorescent+probe+kit/dihydroethidium/10__1016_slash_j__cej__2024__153726-46-14-27
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Image Search Results


Impact of various antioxidants on fresh bull sperm motility and reactive oxygen species (ROS) levels when exposed to different oxidative stress triggers. ( A ) Experimental design to examine the impact of various antioxidants on sperm motility and levels of reactive oxygen species (ROS) under the two oxidative stress inducers. ( B and C ) Changes in sperm viability and motility under H 2 O 2 ( B ) or antimycin (C). ( D - G ) Effect of antioxidants on the percentages of DCFH-DA and mtSOX deep Red positive sperm under H 2 O 2 or antimycin. ( H - J ) Measurement of mitochondrial activity using the JC-1 kit. ( H ) Representative flow cytometric pattern of sperm that was not treated with any drug (control) stained with JC-1. Effect of antioxidants on the percentages of high mitochondrial membrane potential (hMMP) under the treatment of H 2 O 2 ( I ) and antimycin ( J ). H 2 O 2 or antimycin groups were used as controls for statistical analysis ( p < 0.05).

Journal: In Vitro Cellular & Developmental Biology. Animal

Article Title: The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm

doi: 10.1007/s11626-024-00944-w

Figure Lengend Snippet: Impact of various antioxidants on fresh bull sperm motility and reactive oxygen species (ROS) levels when exposed to different oxidative stress triggers. ( A ) Experimental design to examine the impact of various antioxidants on sperm motility and levels of reactive oxygen species (ROS) under the two oxidative stress inducers. ( B and C ) Changes in sperm viability and motility under H 2 O 2 ( B ) or antimycin (C). ( D - G ) Effect of antioxidants on the percentages of DCFH-DA and mtSOX deep Red positive sperm under H 2 O 2 or antimycin. ( H - J ) Measurement of mitochondrial activity using the JC-1 kit. ( H ) Representative flow cytometric pattern of sperm that was not treated with any drug (control) stained with JC-1. Effect of antioxidants on the percentages of high mitochondrial membrane potential (hMMP) under the treatment of H 2 O 2 ( I ) and antimycin ( J ). H 2 O 2 or antimycin groups were used as controls for statistical analysis ( p < 0.05).

Article Snippet: Photooxidation-resistant dichloro-dihydro-fluorescein diacetate (DCFH-DA) is a total ROS (H 2 O 2 , superoxide anion, and hydroxyl radical) probe (R253; DOJINDO LABOLATORIES Co., Ltd., Kumamoto, Japan).

Techniques: Activity Assay, Control, Staining, Membrane

The effect of various antioxidants on frozen-thawed bull sperm motility and ROS levels. ( A ) Experimental design to examine the impact of PQQ, vitamin C, and ergothioneine on frozen-thawed bull sperm motility and ROS levels. Various antioxidants were included in all thawing, washing, and incubation processes using HTF medium as the basic medium. ( B and C ) Effect of antioxidants on the percentages of DCFH-DA ( B ) and mtSOX deep Red ( C ) positive sperm. ( D ) Changes in frozen-thawed sperm viability and kinetics patterns with antioxidants. Asterisks represent significant differences from the control (Ctrl; *: p < 0.05, **: p < 0.01, ***: p < 0.001).

Journal: In Vitro Cellular & Developmental Biology. Animal

Article Title: The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm

doi: 10.1007/s11626-024-00944-w

Figure Lengend Snippet: The effect of various antioxidants on frozen-thawed bull sperm motility and ROS levels. ( A ) Experimental design to examine the impact of PQQ, vitamin C, and ergothioneine on frozen-thawed bull sperm motility and ROS levels. Various antioxidants were included in all thawing, washing, and incubation processes using HTF medium as the basic medium. ( B and C ) Effect of antioxidants on the percentages of DCFH-DA ( B ) and mtSOX deep Red ( C ) positive sperm. ( D ) Changes in frozen-thawed sperm viability and kinetics patterns with antioxidants. Asterisks represent significant differences from the control (Ctrl; *: p < 0.05, **: p < 0.01, ***: p < 0.001).

Article Snippet: Photooxidation-resistant dichloro-dihydro-fluorescein diacetate (DCFH-DA) is a total ROS (H 2 O 2 , superoxide anion, and hydroxyl radical) probe (R253; DOJINDO LABOLATORIES Co., Ltd., Kumamoto, Japan).

Techniques: Incubation, Control

Significant decrease in viability and an increase in ROS levels of fresh bull sperm during the incubation period. ( A ) Tracks of sperm incubated for 0 and 4 h were determined using the CASA system. Red arrows indicate the aggregated sperm, which is the cause of overestimating CASA parameters because it lowers the number of dead sperm. ( B ) Changes in fresh sperm motility single counts implying sperm viability and in kinetics patterns with incubation time. ( C and D ) Time-dependent changes in total ROS in the cytoplasm or mitochondrial superoxide level of fresh bull sperm detected using DCFH-DA or mtSOX deep Red, respectively. Flow cytometric patterns and percentage of positive sperm were shown. Different letters represent significantly different groups ( p < 0.05).

Journal: In Vitro Cellular & Developmental Biology. Animal

Article Title: The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm

doi: 10.1007/s11626-024-00944-w

Figure Lengend Snippet: Significant decrease in viability and an increase in ROS levels of fresh bull sperm during the incubation period. ( A ) Tracks of sperm incubated for 0 and 4 h were determined using the CASA system. Red arrows indicate the aggregated sperm, which is the cause of overestimating CASA parameters because it lowers the number of dead sperm. ( B ) Changes in fresh sperm motility single counts implying sperm viability and in kinetics patterns with incubation time. ( C and D ) Time-dependent changes in total ROS in the cytoplasm or mitochondrial superoxide level of fresh bull sperm detected using DCFH-DA or mtSOX deep Red, respectively. Flow cytometric patterns and percentage of positive sperm were shown. Different letters represent significantly different groups ( p < 0.05).

Article Snippet: Photooxidation-resistant dichloro-dihydro-fluorescein diacetate (DCFH-DA) is a total ROS (H 2 O 2 , superoxide anion, and hydroxyl radical) probe (R253; DOJINDO LABOLATORIES Co., Ltd., Kumamoto, Japan).

Techniques: Incubation

The impact of oxidative stress inducers, H 2 O 2 and antimycin, on fresh bull sperm motility and ROS levels. ( A ) Experimental design to investigate the effect of the oxidative stress inducers on fresh bull sperm. ( B ) Representative images of the sperm fluorescence signal with DCFH-DA and mtSOX deep Red. Left : DCFH-DA fluorescence image of sperm treated with H 2 O 2 . Right : mtSOX deep Red fluorescence image of sperm treated with antimycin. ( C ) Flow cytometric patterns of DCFH-DA. ( D ) Percentages of DCFH-DA positive sperm. ( E ) Sperm kinetics changed by H 2 O 2 . ( F ) Flow cytometric patterns of mtSOX deep Red. ( G ) Percentages of mtSOX deep Red positive sperm. ( H ) Sperm kinetics changed by antimycin. Different letters represent significantly different groups ( p < 0.05).

Journal: In Vitro Cellular & Developmental Biology. Animal

Article Title: The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm

doi: 10.1007/s11626-024-00944-w

Figure Lengend Snippet: The impact of oxidative stress inducers, H 2 O 2 and antimycin, on fresh bull sperm motility and ROS levels. ( A ) Experimental design to investigate the effect of the oxidative stress inducers on fresh bull sperm. ( B ) Representative images of the sperm fluorescence signal with DCFH-DA and mtSOX deep Red. Left : DCFH-DA fluorescence image of sperm treated with H 2 O 2 . Right : mtSOX deep Red fluorescence image of sperm treated with antimycin. ( C ) Flow cytometric patterns of DCFH-DA. ( D ) Percentages of DCFH-DA positive sperm. ( E ) Sperm kinetics changed by H 2 O 2 . ( F ) Flow cytometric patterns of mtSOX deep Red. ( G ) Percentages of mtSOX deep Red positive sperm. ( H ) Sperm kinetics changed by antimycin. Different letters represent significantly different groups ( p < 0.05).

Article Snippet: Photooxidation-resistant dichloro-dihydro-fluorescein diacetate (DCFH-DA) is a total ROS (H 2 O 2 , superoxide anion, and hydroxyl radical) probe (R253; DOJINDO LABOLATORIES Co., Ltd., Kumamoto, Japan).

Techniques: Fluorescence

Impact of antioxidants on total ROS and mitochondrial ROS levels in fresh bull sperm under oxidative stress inducers. ( A ) Experimental design to investigate the effect of three antioxidants on induced reactive oxygen species in fresh bull sperm. ( B ) Flow cytometric patterns of H 2 O 2 and antioxidant-treated sperm stained with DCFH-DA. ( C ) Percentages of DCFH-DA positive sperm. ( D ) Flow cytometric patterns of antimycin and antioxidant-treated sperm stained with mtSOX deep Red. ( E ) Percentages of mtSOX deep Red positive sperm. Different letters represent significantly different groups ( p < 0.05).

Journal: In Vitro Cellular & Developmental Biology. Animal

Article Title: The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm

doi: 10.1007/s11626-024-00944-w

Figure Lengend Snippet: Impact of antioxidants on total ROS and mitochondrial ROS levels in fresh bull sperm under oxidative stress inducers. ( A ) Experimental design to investigate the effect of three antioxidants on induced reactive oxygen species in fresh bull sperm. ( B ) Flow cytometric patterns of H 2 O 2 and antioxidant-treated sperm stained with DCFH-DA. ( C ) Percentages of DCFH-DA positive sperm. ( D ) Flow cytometric patterns of antimycin and antioxidant-treated sperm stained with mtSOX deep Red. ( E ) Percentages of mtSOX deep Red positive sperm. Different letters represent significantly different groups ( p < 0.05).

Article Snippet: Photooxidation-resistant dichloro-dihydro-fluorescein diacetate (DCFH-DA) is a total ROS (H 2 O 2 , superoxide anion, and hydroxyl radical) probe (R253; DOJINDO LABOLATORIES Co., Ltd., Kumamoto, Japan).

Techniques: Staining

BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) fluorescent probe, accompanied by the relevant quantitative determination. ( b , d ) Superoxide anion (O 2 − ) production in irradiated BMSCs was monitored with the DHE fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.

Journal: Bioengineering

Article Title: Fe 3 O 4 Magnetic Nanoparticles and Static Magnetic Field Stimulated BMSC-Derived Exosomes Promoted Osteogenesis and Alleviated Oxidative Stress in Irradiated BMSCs Through miR-429/NOG Pathway

doi: 10.3390/bioengineering13040402

Figure Lengend Snippet: BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) fluorescent probe, accompanied by the relevant quantitative determination. ( b , d ) Superoxide anion (O 2 − ) production in irradiated BMSCs was monitored with the DHE fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.

Article Snippet: Similarly, intracellular superoxide anion levels were measured using a Dihydroethidium (DHE) Superoxide Anion Fluorescent Probe Kit (G1904-100T, Servicebio, Wuhan, Hubei, China) in full accordance with the manufacturer’s instructions.

Techniques: Irradiation, Concentration Assay